Journal: Journal of Aerosol Science

Article Title: SARS-CoV-2 in residential rooms of two self-isolating persons with COVID-19

doi: 10.1016/j.jaerosci.2021.105870

Figure Lengend Snippet: rRT-qPCR results of environmental samples from volunteer A's room.

Article Snippet: The PCIS (catalog no. 225–370, SKC Inc., US) was used with a Leland Legacy pump (catalog no. 100–3002, SKC Inc., US) and operated at a flow rate of 9 L/min for 90 min. PTFE filters (25 mm, 0.5 μm pore, catalog no. 225–2708, SKC Inc., US) were used to collect particles of size >2.5 μm, 1–2.5 μm, 0.5–1 μm and 0.25–0.5 μm in the 4 collection stages.

Techniques: Environmental Sampling, Sampling

Journal: Journal of Biological Chemistry

Article Title: DLX5 (Distal-less Homeobox 5) Promotes Tumor Cell Proliferation by Transcriptionally Regulating MYC

doi: 10.1074/jbc.m109.021477

Figure Lengend Snippet: FIGURE 3. DLX5 binds to MYC promoter in vitro and in vivo. A, gel shift assays were performed using recombinant human DLX5 protein and radiolabeled oligonucleotides. DLX5 binding could be competed by excess molar concentrations (25-, 50-, or 250-fold excess) of unlabeled probes, as indicated by the triangles. Supershift was observed by adding a DLX5-specific antibody (-DLX5) into each reaction. The arrow indicates oligonucleotides binding to DLX5. B, ChIP assay carried out using ChIP-grade antibody against DLX5 (or normal goat IgG as a negative control) with HeLa cells transiently transfected with DLX5 or NCI-H322M cells. PCR products represent a portion of the MYC promoter (MYC). The upstream control (Upstream Con) represents a portion of the upstream MYC promoter containing nucleotides 2305 to 2015. The second control (Downstream Con) represents a downstream sequence containing nucleotides 1801–2062. Nucleotide numbers are relative to the P2 transcription start site (1) in the MYC promoter. CON, positive control using total genomic DNA as template; DLX5, PCR products amplified from the ChIP samples mediated by anti-DLX5 antibody; IgG, absence of PCR product corresponding to MYC promoter in sample mediated by IgG.

Article Snippet: Recombinant protein DLX5 (Prosci) was incubated in a reaction containing 1 gel shift binding buffer in a volume of 9 l for 10 min, followed by the addition of 1 l of 32P-labeled duplex oligonucleotide probes corresponding to the MYC promoter region (as shown under “Results”).

Techniques: In Vitro, In Vivo, Gel Shift, Recombinant, Binding Assay, Negative Control, Transfection, Control, Sequencing, Positive Control, Amplification

Journal: Journal of Biological Chemistry

Article Title: DLX5 (Distal-less Homeobox 5) Promotes Tumor Cell Proliferation by Transcriptionally Regulating MYC

doi: 10.1074/jbc.m109.021477

Figure Lengend Snippet: FIGURE 3. DLX5 binds to MYC promoter in vitro and in vivo. A, gel shift assays were performed using recombinant human DLX5 protein and radiolabeled oligonucleotides. DLX5 binding could be competed by excess molar concentrations (25-, 50-, or 250-fold excess) of unlabeled probes, as indicated by the triangles. Supershift was observed by adding a DLX5-specific antibody (-DLX5) into each reaction. The arrow indicates oligonucleotides binding to DLX5. B, ChIP assay carried out using ChIP-grade antibody against DLX5 (or normal goat IgG as a negative control) with HeLa cells transiently transfected with DLX5 or NCI-H322M cells. PCR products represent a portion of the MYC promoter (MYC). The upstream control (Upstream Con) represents a portion of the upstream MYC promoter containing nucleotides 2305 to 2015. The second control (Downstream Con) represents a downstream sequence containing nucleotides 1801–2062. Nucleotide numbers are relative to the P2 transcription start site (1) in the MYC promoter. CON, positive control using total genomic DNA as template; DLX5, PCR products amplified from the ChIP samples mediated by anti-DLX5 antibody; IgG, absence of PCR product corresponding to MYC promoter in sample mediated by IgG.

Article Snippet: Recombinant protein DLX5 (Prosci) was incubated in a reaction containing 1 gel shift binding buffer in a volume of 9 l for 10 min, followed by the addition of 1 l of 32P-labeled duplex oligonucleotide probes corresponding to the MYC promoter region (as shown under “Results”).

Techniques: In Vitro, In Vivo, Gel Shift, Recombinant, Binding Assay, Negative Control, Transfection, Control, Sequencing, Positive Control, Amplification

Journal: Journal of Biological Chemistry

Article Title: DLX5 (Distal-less Homeobox 5) Promotes Tumor Cell Proliferation by Transcriptionally Regulating MYC

doi: 10.1074/jbc.m109.021477

Figure Lengend Snippet: FIGURE 4. DLX5 promotes Jurkat cell proliferation by regulating the expression of MYC. A, expression of MYCproteininJurkatcellstransfectedbyvector(1)orDLX5(2).ThehistograminArepresentsthedensityofthe MYCbandsinimmunoblot.B,realtimeRT-PCRanalysisofMYCmRNAlevelsinJurkatcellstransfectedbyvector and DLX5. C, expression of MYC in Jurkat cells transfected with increasing amounts of DLX5 plasmid. D, real time RT-PCR analysis of MYC mRNA level in Jurkat cells transfected with increasing amounts of DLX5 plasmid. E, cell numbers counted at 0, 24, and 48 h in Jurkat cells transfected with vector (Vec), DLX5, DLX5 non- targeting siRNA (siCon), DLX5 siRNA1 against MYC (siMYC1), DLX5 siRNA2 against MYC (siMYC2), and MYC. F, expression of MYC, DLX5, and -ACTIN in Jurkat cells transfected with Vec (1), DLX5 (2), DLX5 non- targeting siRNA (3); DLX5 siRNA1 against MYC (4), DLX5 siRNA2 against MYC (5), and MYC (6).

Article Snippet: Recombinant protein DLX5 (Prosci) was incubated in a reaction containing 1 gel shift binding buffer in a volume of 9 l for 10 min, followed by the addition of 1 l of 32P-labeled duplex oligonucleotide probes corresponding to the MYC promoter region (as shown under “Results”).

Techniques: Expressing, Transfection, Plasmid Preparation, Quantitative RT-PCR

Journal: Journal of Biological Chemistry

Article Title: DLX5 (Distal-less Homeobox 5) Promotes Tumor Cell Proliferation by Transcriptionally Regulating MYC

doi: 10.1074/jbc.m109.021477

Figure Lengend Snippet: FIGURE 5. DLX5 promotes proliferation of lung cancer cells by regulating the expression of MYC. A, real time RT-PCR analyses of -fold change of DLX5 and MYC mRNA levels in NCI-H322M, NCI-H520, or NCI-H23 lung cancer cells transfected with siRNA against DLX5 (siDLX5; Invitrogen Oligo ID, HSS102810) or non-targeting siRNA (siCON) for 16 h or 24 h. The DLX5 and MYC mRNA levels were normalized against the respective DLX5 and MYC mRNA levels observed in cells transfected with control non-targeting siRNA. B, the same number of cells were transfected with buffer (Normal), non-targeting siRNA (siCON), siRNA1 againstDLX5(siDLX5-1;InvitrogenOligoID,HSS102808),orsiRNA2againstDLX5(siDLX5-2;InvitrogenoligoID,HSS102810)andcounted48haftertransfection. C, cell numbers counted at 48 h in NCI-H322M cells transfected with buffer (NCI-H322M), non-targeting siRNA (siCONTROL), siRNA1 against DLX5 (siDLX5-1), siRNA2 against DLX5 (siDLX5-2), siRNA1 against DLX5 pcDNA3-MYC (siDLX5-1 MYC), or siRNA2 against DLX5 pcDNA3-MYC (siDLX5-2 MYC). D, expres- sion of DLX5, CASPASE 3, and -ACTIN in NCI-H322M cells transfected with buffer (Normal), non-targeting siRNA (siCON), siRNA1 against DLX5 (siDLX5-1), or siRNA2 against DLX5 (siDLX5-2). The arrow points to the bands representing the cleaved form of active CASPASE 3.

Article Snippet: Recombinant protein DLX5 (Prosci) was incubated in a reaction containing 1 gel shift binding buffer in a volume of 9 l for 10 min, followed by the addition of 1 l of 32P-labeled duplex oligonucleotide probes corresponding to the MYC promoter region (as shown under “Results”).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Control